Protocol for Exploring the Role of Irisin in the Enhancement of Spatial Learning Mediated by Aerobic Physical Exercise in Adult Mice

Introduction: Age-related cognitive decline decreases with aerobic physical exercise in many animal models and humans. Irisin, a myokine its’ precursor FNDC5, has increased hippocampal development to improve learning memory. This study aims determine the association between irisin, activity enhancement of spatial adult mice. Methods: Three experimental studies will be conducted explore role FNDC5/irisin learning. Study 1 test ability C57BL/6 mice using Morris Water Maze (MWM) task WT, FNDC5+/- FNDC5-/- strains. 2 changes MWM due exogenous administration hippocampus, specifically dentate gyrus region. 3 effect on irisin levels region task. Anticipated Results: results are expected show that WT perform better compared completing is anticipated can rescue phenotype. PE improves all strains by regulating levels. Discussion: play enhancing neurogenesis synaptic plasticity downstream signalling pathways involved functions. Aerobic enhances this mechanism, resulting improved Conclusion: significantly regulates FNDC5/irisin, which associated